DEVELOPMENT OF A FERMENTED VEGETABLE PRODUCT USING A MIXED STARTER INCLUDING LACTIC ACID BACTERIA AND ACETIC ACID BACTERIA

Abstract

Vegetables are an indispensable valuable food product. Fermentation of vegetables is one of the widely used methods for extending the shelf life and safety of vegetable products, resulting in new products. The use of starter cultures makes it possible to increase the controllability of the fermentation process and obtain new types of products. 27 isolates of lactic acid bacteria and 5 isolates of acetic acid bacteria were selected from fermented vegetables. Microorganisms with better biotechnologically significant indicators were used for creating associations and screened in the fermentation of mixed vegetable product experiments. A fermented vegetable product with high sensory properties from white cabbage, carrots, and bell peppers was obtained using a selected association. The association microorganisms were identified by Sanger sequencing as Lacticaseibacillus sp. 1K7, Lacticaseibacillus paracasei Bel1, Pediococcus pentosaceus 2K3 and Acetobacter pasteurianus 2-6. Volatile organic compounds analysis of the squeezed juice by gas chromatography with mass spectrometric detection showed the presence of organic acids, in contrast to the spontaneously fermented sample. The spectrum of volatile compounds of the product made with the starter was wider when A. pasteurianus 2-6 were included in the association. The obtained results indicate the possibility of improving the biotechnologically valuable indicators of fermented vegetables by using acetic acid bacteria in the composition of the starter.