PHYTOPATHOGENIC FUNGI OF THE GENUS PENICILLIUM SP. ON TULIPS
DOI:
https://doi.org/10.53729/MV-AS.2023.04.14Abstract
Abstract
Plant disease is a very complex pathological process. Phytopathogenic fungi occupy the main place among pathogenic microorganisms in plants. One of them is the causative agent of fungal disease – penicillosis. The purpose of the work is to determine the distribution and species composition of fungi of the genus Penicillium sp. The bioecological features of the causative agent of the disease affecting tulips grown in open and greenhouse conditions have been revealed. In related tulip species, surface infection prevailed over subepidermal infection. These types of fungi had a significant impact on the quality of the bulb, significantly reducing the germination and viability of the affected. The most affected exclusive variety was the type of Tulipa Dynasty and the most stable-Tulipa Delta Storm.
The spread and development of the disease (P%) were determined by the wet chamber method, cultivation of Penicillium sp on PDA (potato-dextrose agar), microscopic analysis, as well as PCR (polymerase chain reaction). The results of PCR analysis showed that tulips in greenhouse conditions are mainly affected by the fungi Talaromyces variabilis (Penicillium variabile), the prevalence of penicillosis averaged about 7-9% in the period from 2021 to 2022.
As a result of the comparison, the type of strain was identified. The relevance of the names of fungi identified as a result of identification, and the correct spelling of the names of species was checked using the nomenclature of the Mycobank information base.