IN VITRO PLANT CELL CULTURE AJUGA TURKESTANICA

M.К. Tynykulov; А.B. Akhmetova; А.В. Abzhalelov; Sh.Е. Arystanova; А.U. Utaubayeva; Zh.N. Ualiakhmetova; А.U. Tuyakbayeva; А.Zh. Temirkhanov

doi:10.53729/MV-AS.2024.02.10

Authors

M.К. Tynykulov LN Gumilyov Eurasian national university
А.B. Akhmetova al-Farabi Kazakh National University
А.В. Abzhalelov L.N. Gumilyov Eurasian National University
Sh.Е. Arystanova L.N. Gumilyov Eurasian National University
А.U. Utaubayeva M. Utemisov West Kazakhstan University
Zh.N. Ualiakhmetova M. Utemisov West Kazakhstan University
А.U. Tuyakbayeva L.N. Gumilyov Eurasian National University
А.Zh. Temirkhanov LLP «Republican collection of microorganisms»

DOI:

https://doi.org/10.53729/MV-AS.2024.02.10

Keywords:

Ajuga turkestanica, endemic, in vitro, suspension, cultivation

Abstract

The article presents the growth rates of cells of new lines obtained by inoculation-strains of the culture of the endemic plant Ajuga turkestanica. Scientific research was conducted in 2022 in the laboratory of biology of cultured cells of the Institute of Plant Physiology named after K.A. Timiryazev. The purpose of the work: to obtain new tissue and cell culture lines in vitro of the endemic Ajuga turkestanica plant, as well as to study the possibility of regulating culture growth and biosynthesis of secondary metabolism products by the influence of various factors. The objects of the study are suspension cultures of Ajuga turkestanica cells. Source material: callus strain Ajuga turkestanica (Regel) Briq. At21, received in 2016, as well as ready-made frozen collection bases from the cryobank of the Timiryazev IGF of the Russian Academy of Sciences. Research methods: Obtaining a suspension of cells from callus tissue; cultivation of suspension cultures; determination of growth characteristics of the culture: the content of dry and raw biomass in a liter of medium, the concentration of cells in the medium, the viability of the culture. The results of the evaluation of the growth characteristics of suspension crops were obtained. Practical significance: the obtained suspension cultures can be used for various biotechnological studies, such as the production of biologically active substances, the study of secondary metabolism, the development of biotechnological methods for the production of medicines.