SELECTION OF CARBON FEED SOURCES FOR CULTIVATION OF CELLULOLITIC BACTERIA

Abstract

The main fodder and legume crops in Kazakhstan are alfalfa and sweet clover, but when growing them, there is a problem of low seed germination. To increase the germination and productivity of these crops, the most promising is the use of a biological method based on the use of cellulolytic bacteria. To create a biofertilizer based on these bacteria, a high profitability of its production is required. One of the ways to reduce the cost of fertilizer production is the selection of optimal nutrition sources to increase the yield of bacterial biomass. The purpose of this study was to select the optimal carbon source for the cultivation of cellulolytic bacteria and to obtain an increased yield of biomass with a high activity of cellulase enzymes. The selection of sources of carbon nutrition for three strains of cellulolytic bacteria was carried out. It was established that the maximum accumulation of bacterial biomass was noted on the medium with wheat straw. The accumulation of biomass on the medium with lactose was somewhat lower and amounted to 2.7-2.9 g/l, but the activity of cellulases when growing bacteria on these carbon sources was almost equal. However, when increasing the biomass of bacteria in production-type fermenters, wheat straw cannot be used as a carbon source. Therefore, for the cultivation of cellulolytic bacteria, lactose was selected as a carbon source.